无论父母是否健在,都不可以申请姐姐团聚移民:
http://www.cic.gc.ca/english/immigrate/sponsor/relatives-apply-who.asp#who_cannot
Who cannot be sponsored
Other relatives, such as brothers and sisters over 18, or adult independent children cannot be sponsored. However, if they apply to immigrate under the Skilled Worker Class, they may get extra points for adaptability for having a relative in Canada.